Abstract:

Objective　To investigate the expression of miRNA150-5p in papillary thyroid carcinoma (PTC) and its utility in differentiating PTC from benign thyroid nodules (BTN). Methods　In this study, 43 PTC patients and 19 BTN patients diagnosed in Luoyang Central Hospital affiliated to Zhengzhou University from October 2021 to January 2022 were included. The clinical data of the two groups were statistically analyzed. The real time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miRNA150-5p in the PTC tissue, paracancerous tissue, and BTN tissue, and were verified through The Cancer Genome Atlas (TCGA) RNA-Seq database. The univariate and multivariate logistic regression models were used to analyze the influencing factors of PTC. The value of miRNA150-5p in the differential diagnosis of thyroid nodules was analyzed by the receiver operating characteristic curve (ROC). Results　The expression of miRNA150-5p in the PTC tissue was lower than those in the matched paracancerous tissue and BTN tissue (both P<0.05). The age of the PTC group was lower than that of the BTN group (both P<0.05). The thyroid stimulating hormone (TSH) level of the PTC group was significantly higher than that of the BTN group (P<0.05). Univariate and multivariate logistic regression analyses showed that the higher the expression of miRNA150-5p, the lower the risk of PTC (OR=0.980, P<0.05). The ROC analysis showed that the area under the curve (AUC) was 0.797; the sensitivity, specificity, and accuracy were 67.44%, 89.47%, and 74.19%, respectively. Conclusion　MiRNA150-5p is significantly under-expressed in PTC tissue and has good diagnostic efficacies for benign and malignant thyroid nodules, so it may serve as a potential biomarker in the diagnosis of PTC.

Key words:

MiRNA, MiRNA150-5p, Papillary thyroid carcinoma, Thyroid nodule

摘要：

目的　探讨miRNA150-5p在甲状腺乳头状癌（papillary thyroid carcinoma，PTC）中的表达及其在鉴别PTC与良性甲状腺结节（benign thyroid nodule，BTN）中的作用。方法　纳入2021年10月至2022年1月在郑州大学附属洛阳中心医院确诊的43例PTC患者和19例BTN患者，对两组患者的临床资料进行统计学分析。采用定量实时聚合酶链式反应（qRT-PCR）检测PTC组织、癌旁组织及BTN组织中miRNA150-5p的表达水平，并在癌症基因组图谱（TCGA）RNA-Seq数据库中进行验证。使用单因素和多因素logistic回归模型分析PTC的影响因素。采用受试者工作特征曲线（ROC）分析miRNA150-5p对于甲状腺结节性质的鉴别诊断价值。结果　PTC组织中miRNA150-5p的表达量低于配对癌旁组织和BTN组织（均P<0.05）。PTC组患者年龄低于BTN组（P<0.05）；PTC组促甲状腺激素（TSH）水平明显高于BTN组（P<0.05）。单因素和多因素logistic回归分析表明，miRNA150-5p表达量越高，发生PTC的风险越小（OR=0.980，P<0.05）。ROC分析显示，曲线下面积（AUC）为0.797，灵敏度为67.44%，特异度为89.47%，准确度为74.19%。结论　miRNA150-5p在PTC组织中显著低表达，对良性和恶性甲状腺结节具有良好的诊断效能，可作为诊断PTC的潜在生物标志物。

关键词:

miRNA, miRNA150-5p, 甲状腺乳头状癌, 甲状腺结节