Upregulation of NCAPG gene expression promotes lung adenocarcinoma cell
proliferation and is associated with poor clinical prognosis

doi:10.3760/cma.j.issn.1007-1245.2022.03.019

Abstract

Abstract: Objective　To investigate the expression of the NCAPG gene in lung adenocarcinoma tissue and its relevance to the clinical prognosis and its molecular mechanisms. Methods　From January 2017 to December 2019, the samples of the tumor tissue and the nearby normal tissue were collected from 110 patients with lung adenocarcinoma confirmed and surgically treated at Lianyungang Second People's Hospital. The relationship between NCAPG gene expression and the prognosis of lung adenocarcinoma patients was analyzed using the TCGA database. The NCAPG expressions in the 110 adenocarcinoma samples and normal samples were assessed using immunohistochemistry (IHC). The cell lines stably knocking down NCAPG in lung adenocarcinoma were obtained using a lentivirus-mediated shRNA. CCK-8 and colony formation experiments were used to investigate the proliferative capacity of NCAPG on lung adenocarcinoma cell lines (A549, H1975). The co-expressed genes of NCAPG were explored by protein-protein interaction (PPI) and co-expression analysis, and validated using coimmunoprecipitation (CO-IP). The differences in OS and DFS were judged by the Log-rank test. The correlation between the clinical pathological characteristics and NCAPG expression in the 110 patients was evaluated by chi-square test. Results　NCAPG was upregulated in the TCGA lung adenocarcinoma database and in the 110 samples of lung adenocarcinoma tissue, and was associated with tumor size (P=0.048) and clinical stage (P=0.021) in lung adenocarcinoma patients. Furthermore, the knockdown of NCAPG inhibited lung adenocarcinoma cell proliferation and growth. Mechanistically, we further found that CDK1 had an expression correlation with NCAPG and binds with each other, jointly promoting the progression of lung adenocarcinoma. Conclusion　NCAPG can be used as a marker of poor prognosis in patients with lung adenocarcinoma and as a potential target for the treatment of lung adenocarcinoma.

Key words: Non-SMC condensin I complex subunit G (NCAPG), Proliferation, Lung adenocarcinomas, Cyclin-dependent kinase 1, Therapeutic target

摘要： 目的　探究非SMC凝聚素I复合亚基G（NCAPG）基因在肺腺癌组织中的表达以及与临床预后的相关性和其分子机制。方法　2017年1月至2019年12月在连云港市第二人民医院确诊并接受手术治疗的肺腺癌患者的肿瘤组织及邻近正常组织110份。使用TCGA数据库分析NCAPG基因表达与肺腺癌患者预后的关系。使用免疫组织化学（IHC）评估NCAPG在110个肺腺癌组织和正常组织中的表达。使用慢病毒介导的shRNA获得稳定敲低NCAPG的肺腺癌的细胞系。采用CCK-8和集落形成实验研究NCAPG对肺腺癌细胞系（A549，H1975）增殖能力的影响。通过蛋白质-蛋白质相互作用（PPI）和共表达分析探究NCAPG的共表达基因，并使用免疫共沉淀（CO-IP）验证。采用Log-rank方法判定总生存期（OS）和无病生存期（DFS）的统计差异，采用卡方检验评估110例患者的临床病理特征和NCAPG的表达之间的相关性。结果　NCAPG在TCGA肺腺癌数据库和110个肺腺癌组织中上调，并且与肺腺癌患者的肿瘤大小（P=0.048）和临床分期（P=0.021）有关。此外，敲低NCAPG抑制了肺腺癌细胞增殖和生长。机制上，我们进一步发现细胞周期蛋白依赖性激酶1（CDK1）与NCAPG具有表达相关性，并相互结合，共同促进了肺腺癌的进展。结论　NCAPG可以用作肺腺癌预后不良的标志物和肺腺癌治疗的潜在靶点。

关键词: 非SMC凝聚素I复合亚基G, 增殖, 肺腺癌, 细胞周期蛋白依赖性激酶1, 治疗靶点

Yang Yang, Liao Yingying, Chen Futao. Upregulation of NCAPG gene expression promotes lung adenocarcinoma cell proliferation and is associated with poor clinical prognosis [J]. International Medicine and Health Guidance News, 2022, 28(3): 371-379.

杨飏, 廖莹莹, 陈福涛. NCAPG基因的表达上调促进肺腺癌细胞增殖并与不良临床预后相关[J]. 国际医药卫生导报, 2022, 28(3): 371-379.

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Upregulation of NCAPG gene expression promotes lung adenocarcinoma cell proliferation and is associated with poor clinical prognosis

NCAPG基因的表达上调促进肺腺癌细胞增殖并与不良临床预后相关

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